A description of the technique for nuclear transplantation in Xenopus laevis.

نویسندگان

  • T R ELSDALE
  • J B GURDON
  • M FISCHBERG
چکیده

INTRODUCTION A METHOD by which nuclei can be successfully transplanted into Amphibian eggs was first worked out by Briggs & King (1952) for the eggs of Ranapipiens. Xenopus laevis is an atypical Anuran since its eggs can be obtained throughout the year, and the resulting embryos can be reared to maturity within 12 months under laboratory conditions. Because of these advantages we have used Xenopus for nuclear transplantation experiments. Though the principle of Briggs & King's technique has been followed, differences between the eggs of Rana and Xenopus have made it necessary to modify their technique before it can be satisfactorily applied to the eggs of Xenopus. The purpose of this publication is first to give technical details of these modifications, and secondly to discuss the extent to which they might affect a direct comparison of the results of transplantations in Rana and Xenopus.

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عنوان ژورنال:
  • Journal of embryology and experimental morphology

دوره 8  شماره 

صفحات  -

تاریخ انتشار 1960